Resource: Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology - 14 Engineered chromophore genes (meffRed, eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple and gfasPurple colors)

Primer Design

Step 1: Get super confused

**Step 2: Open Benchling

2.1 AmilCP Gene Region**

Add gene primers guidelines

• Length of 18-24 bases
• 40-60% G/C content
• Start and end with 1-2 G/C pairs
• Melting temperature (Tm) of 50-60°C
• Primer pairs should have a Tm within 5°C of each other
• Primer pairs should not have complementary regions

Used the primer3 tool in benchling/ http://primer3.ut.ee/

1. One set of amplicons copy the region of the amilCP gene that precedes the chromophore, including the transcription promoter and translation ribosome-binding site (RBS).