Part 1 - In silico design of Lycopene production

For this week's remote experiment, we studied Lycopene production in E. coli. Lycopene is the red pigment found in tomatoes. It can be produced in cells using the 3-enzyme pathway that converts fructose into our desired molecule.

We used pAC-LYC plasmid to produce Lycopene and pAC-BETA to produce beta-carotene.

Part 2: Identify a cool gene

For my final project is to Fabrication of 3D printed microfluidic device making nanodroplets for drug deliveryand mRNA Vaccine of COVID-19. In this way , I have to identity the gene relate to the COVID19 Virus. In order to do this, I need to produce the spike protein in E. coli.

To design a vector that could express this protein in E. coli, I first looked up the SARS-CoV-2 spike protein sequence in Uniprot and used benchling to do the back translation and codon optimization.

Part 3: If you have the capability of Gingko Bioworks foundry

Design a biosynthetic route to produce a small molecule in an organism. See the class slides for tools and examples. Choose a target compound and answer the following questions:

1. What chassis and pathway will you use?